advocated as a routine fixative by Carleton (1926) andbyLendrum(1941) Its useis nowpromotedby the Association of Clinical Pathologists (1966) Other fixatives containing mercuric chloride such as Zenker's Susa's and Helly's solutions are also incommonuse Since 1947 histopathologists in Dundee have routinely usedformol-corrosive solution paraffin method Later Helly's fluid wasemployedasthe fixative and the eyes were cut after fixation as for the previous method By this latter procedure the inner portions of the eye did not collapse since the vitreous had coagulated during fixation The sections were stained with haemalum and eosin and these stains proved adequate in most cases

Helly's fluid fixation — The Open Lab Book v1 0

Fixation using Helly's fluid To prepare stock solution add 1l distilled water to suitable container Add 25g potassium dichromate Add 50g mercuric chloride Add 10g sodium sulphate and mix thoroughly Store stock solution until required Make up fixative just before use Add 5ml formalin (40% aqueous solution of formaldehyde) to 100ml stock

temperature (except those in Helly's fixative which were fixed 6 hr) washed by the proce dure appropriate for the fixative dehydrated in ethanol and embedded paraffin Serial sections 5 p in thickness and three to four sec tions per slide were used Peroxidase-labeled Antibody Preparation

The object in using Helly's fluid as a fixative almost exclusively was to preserve the cytoplasmic granules both albuminoid and lipoid that is blood-cell granules and mitochondria for subsequent differen tial staining with the Giemsa stain and with iron hematoxylin Paral lel series were prepared with these two stains with the expectation

employed were Helly's modification of Zenker's fluid Flemming-without-acetic acid Heidenhain's Susa fixative and after the molluscs had been fed on iron saccharate Bouin's fluid with ammonium sulphide added * thic Sectionk and were s were cut 6 commonly stained with Heidenhain's iron hsematoxylin but some with Mallory's stain

Helly's fluid / Zenker-formol fixative / formol-Zenker solution: a histologic fixative consisting of Zenker's fluid in which the glacial acetic acid is replaced by formalin The concentration of formalin used varies between 5 and 10% : the most widely used formula consists of 9 parts Zenker stock solution and 1 part neutral formalin ( Zenker

HOLLANDE'S FIXATIVE PURPOSE

HOLLANDE'S FIXATIVE PURPOSE: Primary fixative for G I and prostate biopsies This fixative gives a brilliant hematoxylin and eosin stain it softens the tissues eliminating cracked brittle sections The picric acid will lyse out the red blood cells Lillie states it is a good fixative

Whole oysters were placed into Helly's fixative for 15-30 minutes removed and sagittally sectioned along 95% of the midline and returned to the fixative overnight for 16 to 24 hours Oysters were sectioned transversely through the body mass during final trimming Sections were cut adjacent to the pericardial cavity oriented towards the

***** Helly's Fluid 2 5 gm Potassium Dichromate 5 0 gm Mercuric Chloride 1 0 gm Sodium sulfate 100 ml distilled water 5 ml Formalin ***** Hematoxylin Harris' 0 5 gm Hematoxylin 20 gm Aluminum Ammonium Sulfate 100 ml distilled water 0 5gm Mercuric Oxide Boil the hematoxylin and ammonium alum together in distilled water ( use a large container)

Whole oysters were placed into Helly's fixative for 15-30 minutes removed and sagittally sectioned along 95% of the midline and returned to the fixative overnight for 16 to 24 hours Oysters were sectioned transversely through the body mass during final trimming Sections were cut adjacent to the pericardial cavity oriented towards the

Helly's Fluid Also known as Zenker-formol this is a modification of Zenker's fluid in which formalin is substituted for acetic acid The concentration of formalin used varies between 5 and 10 per cent This is an excellent cytological and tissue fixative H E (Hematoxylin and Eosin Stain)

Zenker's fixative is a rapid-acting fixative for animal tissues It is employed to prepare specimens of animal or vegetable tissues for microscopic study It provides excellent fixation of nuclear chromatin connective tissue fibers and some cytoplasmic features but does not preserve delicate cytoplasmic organelles such as mitochondria Helly's fixative is preferable for traditional dye

Fixation: Formol Zenker (Helly's) Solution for 4 -6 hours Embedding Schedule: 1 Wash 1 hour in running water 2 Decalcify overnight if necessary (until bony button is soft to t he prick of a needle) in Formic Acid 5% (F-92) Cut off cortical bone and wash gently one hour in running water 3

Used as the stock solution for Zenker's solution and Helly's solution Zenker and Helly stock solution Safety concerns Requires cradle to grave tracking If zinc formalin is to be followed by a neutral fixative such as phosphate buffered formalin the tissue must be washed between reagents as a precipitate can form on the tissue

(Module Name) Course Catalogue 2019

4-bouin s solution 5-Zenker s solution c 6- Helly s Carnoy's solutions With the advantages disadvantages of each solution functions -The process of (washing) the specimens Definition aim the solutions used the time -Dehydration: definition aim the solutions used the time

Helly's fluid / Zenker-formol fixative / formol-Zenker solution: a histologic fixative consisting of Zenker's fluid in which the glacial acetic acid is replaced by formalin The concentration of formalin used varies between 5 and 10% : the most widely used formula consists of 9 parts Zenker stock solution and 1 part neutral formalin ( Zenker

Fixative is pumped into the organ and fixes the tissue through the outside and inside of the organ Immersion: It is a method wherein the samples are dissected out and immersed in fixative of volume about 20 times greater than the volume of tissue Immersion fixation fixes from the outside and

11 06 2020Helly's fixative is recommended for the conventional cytogenetic analysis requiring PAS reaction to visualize the developing acrosome After PAS slides must be counterstained with Mayer's hemallume dehydrated in 70–90–100 ethanol and permanently mounted Helly's fixative does not appear appropriate for the subsequent application of

9 Must To Know In Histopathology [vlr0kjm0qplz] Germ layers Categories of tissues Covering Epithelia Simple squamous Simple cuboidal Simple columnar Stratified squamous Stratified columnar Pseudostratified columnar Glandular Epithelia Exocrine glands

6 FIXATIVE CAN INCLUDE: Helly's Zenker's and Boulin's: 7 WHEN DEALING WITH PICRIC ACID: Always keep the picric acid wet: 8 A TEST FOR DECALCIFICATION COULD BE: K ion determinations: 9 THE MOST COMMON FORM OF EMBEDDING MEDIA IS: Paraffin wax: 10 WHEN DECALCIFYING BONE THE VOLUME OF FLUID USED PER VOLUME OF TISSUE IS: 20x: 11 PUTREFACTION IS

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