toluidine blue staining procedure

Many stain options and staining methods ("schedules") for plant tissues have been described previously (Johansen 1940 Jensen 1962 O'Brien and McCully 1981 Sylvester and Ruzin 1994 Ruzin 1999) Here we present a straightforward inexpensive protocol that uses Toluidine Blue O (TBO) to stain A modified toluidine blue O staining technique for Pneumocystis carinii is described An easily prepared sulfation reagent made with sulfuric and acetic acids was used The stain can be employed for bronchoalveolar lavages and lung tissue touch preparations

A study to determine the acceptability in patients and

The adoption of this procedure for employees in a large retail chain in the UK has enabled an investigation into the use of the dye The objective of the study was to determine the acceptability of toluidine blue in aiding the screening for oral cancer in regular attending dental patients who are at high-risk A two-stage study design was

procedure when toluidine blue solution will stain bacteria Method Histology 1) Sections to water 2) Toluidine Blue O 1 - 2 minutes 3) Wash in distilled water 4) Cover directly with coverslide Note: to avoid metachromasia reduction in permanent preparations first dry with filter paper then in air and successively clear in xylene Microbiology

Staining is a technique used to enhance contrast in samples generally at the microscopic level Stains and dyes are frequently used in histology (the study of tissue under the microscope) and in the medical fields of histopathology hematology and cytopathology that focus on the study and diagnoses disease at a microscopic level Stains may be used to define biological tissues (highlighting

10 Oct 2008 hello all could someone please share with me the recipe/protocol for Toluidine blue stain (Sterchi and Eurell)? I want to try a combination B using toluidine blue stain (simpler cheap safe reliable and quicker) Toluidine blue stain can be purchased in powder form from suppliers such as 14 Nov 1998 1 g toluidine blue 1 g sodium borate 100 ml dH2O 2

Staining methods / Staining of nerve tissue HE staining This is a standard staining method used in pathology Typically the cytoplasm of cells is eosinophilic (acidophilic) and is stained red whereas the nuclei and nucleoli are "hematoxylinophilic" (basophilic) and are stained blue

Tzanck smear

Sep 20 2015The slide is stained The choice of stain depends on the sample and diagnostic possibilities Giemsa stain Giemsa stain is poured over the slide After 15 minutes the slide is washed with sterile water Cell cytoplasm stains blue Nuclei stain purple/red/pinkish Toluidine blue Toluidine blue is more rapid as it only requires staining for

In the present study a new staining method was performed by using Toluidine blue stain and compared with the routinely practiced Leishman stain method Toluidine blue method correlated well with traditional Leishman method There are no previous literatures regarding the use of Toluidine blue method for the detection of malaria parasite

Toluidine blue staining: the TB staining test was performed using the TBlue oral lesion marking system manufactured by Zila Inc (Phoenix Arizona) The staining procedure was carried out according to Mashberg recommendations 8 9 The oral rinsing protocol was:

Toluidine Blue Staining Solution: 0 5% Aqueous Toluidine blue O (Fisher T- 161 ) ----- 1 g Distilled water ----- 200 ml Mix to dissolve Procedure: 1 Deparaffinize and hydrate sections to distilled water TOLUENE 5 minutes TOLUENE 5 minutes ethanol 2 minutes ethanol 2 minutes 90% ethanol 2 minutes 70% ethanol 2 minutes

Vital staining is included under the category of staining with dyes is a procedure where living cells take up certain dyes which selectively stain some elements in the cells like mitochondria lipid vesicles lysosomes etc [4] Toluidine blue staining of oral epithelium will not interfere with histologic staining or

BIOPSY PROCEDURES Technique: We almost always use open biopsy procedure to obtain muscle Open biopsy strongly indicated for disorders with patchy pathology e g polymyositis Do not use cautery sutures or clamps Some laboratories use needle for many biopsies to minimize trauma May miss patchy or epimysial pathology

Jan 18 2014Flow chart of volutine granule staining procedure Mechanism Albert stain is basically made up of two stains that is Toluidine blue' O' and Malachite green The pH of Albert stain is adjusted to 2 8 by using acetic acid As we know cytoplasm of cell has neutral pH so the pH of Albert stain is acidic for cytoplasm

Toluidine blue Toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic tissue components It stains nucleic acids blue and polysaccharides purple and also increases the sharpness of histology slide images Used in renal pathology and neuropathology 12 Major Uses of supravital staining in laboratory 1 Reticulocytes

Clinical Guide: Toluidine Blue Dye

Jun 24 2009Toluidine blue in the detection at autopsy of perineal and perianal victims of sexual abuse (Bays Lewman 1992) Toluidine blue in the detection of perineal lacerations in pediatric and adolescent sexual abuse victims (McCauley et al 1986) Use of toluidine blue for documentation of traumatic intercourse (Lauber Souma 1982)

* formaldehyde (10%) Toluidine blue * dH20 (35%) * 0 05% in dH20 Safranin Recipes for reagents used in specimen preparation and staining Bios 193 the paraffin infiltrate cell walls the final stage of infiltration can be conducted in a vacuum oven at approximately 20 cm Mercury Once completely infiltrated with liquid paraffin tissues are

Toluidine Blue Working Solution (pH 2 0~2 5): Toluidine blue stock solution ----- 5 ml 1% Sodium chloride pH 2 3 ----- 45 ml Mix well The pH should be around 2 3 and less than 2 5 Make this solution fresh and discard after use pH higher than 2 5 will make staining less contrast Procedure: 1

Vital staining is included under the category of staining with dyes is a procedure where living cells take up certain dyes which selectively stain some elements in the cells like mitochondria lipid vesicles lysosomes etc [4] Toluidine blue staining of oral epithelium will not interfere with histologic staining or

procedure when toluidine blue solution will stain bacteria Method Histology 1) Sections to water 2) Toluidine Blue O 1 - 2 minutes 3) Wash in distilled water 4) Cover directly with coverslide Note: to avoid metachromasia reduction in permanent preparations first dry with filter paper then in air and successively clear in xylene Microbiology

TOLUIDINE BLUE O excellent quality manufacturer exporter and supplier A metachromatic dye suitable for a wide variety of histological staining procedures Toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic tissue components thereby staining

toluidine blue O because the stain gives better definition of chromosome structure Using a vial for staining reduces the risk of spillage A common mistake is that students mix up the tip and the non-dividing end of the root Heating with a mixture of acetic orcein stain and HCl is a

Basophils purple to dark blue nucleus dark purple almost black large granules Lymphocytes dark purple to deep bluish purple nuclei sky blue cytoplasm Platelets violet to purple granules REFERENCES Conn J I Biological Stains 8th ed Williams and Wilkins Co Baltimore 1969 Clark G (ed ) Staining Procedures Williams and Wilkins Co

Fig 1: (a) Normal brain tissue as positive control Note the distribution of the stain in the nucleolus perikaryon and dendrites (b): Different size ganglion cells in non-HD case (Cresyl Violet x400) - Toluidine blue stain and crystal violet stain versus HE stain in the Diagnosis of Hirschsprung's Disease: A Study in Sulaimani City in Kurdistan/Iraq

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